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Experimental investigation of microbial growth in subsurface environment

Essa, Mohammad Hussein (1993) Experimental investigation of microbial growth in subsurface environment. Masters thesis, King Fahd University of Petroleum and Minerals.


Arabic Abstract


English Abstract

An experimental investigation was conducted to quantify the permeability reduction caused by enhanced biological growth in a porous media. Studies were conducted using columns packed with sand of three different sizes (0.2, 0.3, and 0.4 mm). Phenol was used as a growth substrate. The study involved three distinct Phases with three different concentrations of phenol (15, 50 and 100gm/l). Variations in piezometric head, substrate concentration, and biomass measured as volatile solids, were monitored in space and time. The reductions in permeability were found to be 78% for the coarse, 93% for medium size, and 93.7% for fine sand in Phase-I; Similarly in Phase-II, the reductions were 88.4% for coarse sand, and 94.6% for medium size sand. Finally, in Phase-III, the reductions were 95.5% for medium size, and 96.8% for fine sand. For aerobic sand media, volatile solids can be described as a function of influent substrate concentration respectively. The modified Kozeni-Carman equation was used to estimate the thickness of biological film, knowing the biofilm affected permeability. The estimated values of the film thickness varied from 0.06 mm to 0.09 mm. A good correlation was observed between porosity and biofilm thickness in low range values of thickness.

Item Type:Thesis (Masters)
Date:July 1993
Date Type:Completion
Subjects:Civil Engineering
Divisions:College Of Engineering Sciences > Civil Engineering Dept
Creators:Essa, Mohammad Hussein
Committee Advisor:Farooq, Shaukat
Committee Members:Nakhla, Girgis F. and Khondaker, A. Nasser
ID Code:9864
Deposited By:KFUPM ePrints Admin
Deposited On:22 Jun 2008 16:50
Last Modified:25 Apr 2011 09:29

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